enzyme linked immunosorbent assay elisa kits  (Elabscience Biotechnology)

Journal: International Journal of Molecular Sciences

Article Title: Exploring the Anti-Inflammatory Activity of the Heat-Processed Gynostemma pentaphyllum Extract (Actiponin ® ) in RAW264.7 Cells and Carrageenan-Induced Rat Models

doi: 10.3390/ijms26189145

Figure Lengend Snippet: Effects of AP, DA, and DB on PGE2 production and COX-2 expression in LPS-stimulated RAW264.7 cells. The cells (2 × 10 6 cells) are cultured in 100 mm dishes and treated with AP, DA, and DB for 4 h, then incubated with LPS (500 ng/mL) for another 20 h. PGE2 accumulated in the culture medium is analyzed using PGE2 ELISA; ( A ) AP, ( B ) DA, ( C ) DB. Protein expression of COX-2 is determined using Western blot analysis; ( D ) AP, ( E ) DA, ( F ) DB. ( G – I ) Quantitative data of ( D – F ) were analyzed using ImageJ software. GAPDH served an internal control. The data are presented as the mean ± SEM of three independent experiments. a–e Mean values designated by different superscript letters are significantly different between groups at p < 0.05, as determined by Duncan’s multiple-range test.

Article Snippet: Prostaglandin E2 (PGE2) enzyme-linked immunosorbent assay (ELISA) kit was purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Expressing, Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Western Blot, Software, Control